منابع مشابه
The preparation and properties of crystalline yeast aldolase.
Yeast aldolase was purified partially by Warburg and Christian (I), who found that it was inhibited by metal-binding agents and could be reactivated by addition of Fe++, Co++, or Zn++ ions. Later, this enzyme was further purified, treated with a chelating agent, and crystallized as a mercury salt (2). In this form, the enzyme was still active when tested in the presence of cysteine and Zn++ ion...
متن کامل[Aldolase].
OH OH Two main classes of aldolases are found in Nature. Type I aldolases activate the nucleophilic partner by forming an imine as an intermediate; this type of enzyme is found in animals and higher plants. Type II aldolases occur in microorganisms and they require R 2 to be a hydroxy group. These enzymes feature a Zn 2+ ion that promotes the reaction by forming a chelate with both the carbonyl...
متن کاملOn the Mechanism of the Effect of Ionic Strength on Crystalline Aldolase Activity.
During the course of studies made with rat liver enzymes, it was found that the activity of aldolase was lower when assayed in the presence of phosphate buffer than with tris(hydroxymethyl)aminomethane buffer in the incubation system (1). A similar effect had been observed with crystalline aldolase from rabbit muscle and was attributed to an enhancement of the enzyme activity (2, 3). However, s...
متن کاملThe carboxyl-terminal structure of rabbit liver aldolase (aldolase B).
The amino acid residues in the carboxyl-terminal region of fructose diphosphate aldolase isolated from fresh rabbit liver are -(Gly,Phe,Leu,Ala,Thr2,Ser2)-Tyr. This differs from the carboxyl-terminal structure in aldolase isolated from tissue which had been frozen and thawed. Amino acid analyses, ultracentrifugation, and digestion with carboxypeptidase suggest that the difference is the result ...
متن کاملALDOB (aldolase B, fructose-bisphosphate)
Other names: ALDB, EC 4.1.2.13, OTTHUMP00000021803 HGNC (Hugo): ALDOB Location: 9q31.1 Local order: Telomeric to the PRG-3 (plasticity related gene 3), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), MRPL50 (mitochondrial ribosomal protein L50) and ZNF189 (zinc finger protein 189) genes. Centromeric to C9orf125 (chromosome 9 open reading frame 125), RNF20 (ring finger protein 20), PP...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)57431-7